Research technician molecular cell biology and immunology
The research group led by Dr. Joke den Haan generates different types of cancer nanovaccines (nanobodies, liposomes) that are targeted to macrophages or dendritic cells. You will work in an international team of young and enthusiastic researchers, who have expertise in molecular biology, biochemistry, and immunology. This project is funded by a NWO Open technology grant. Our research group is part of both the Cancer Center Amsterdam and the Amsterdam Institute for Immunology and Infectious Diseases.
Vaccines can be used to activate the immune system to eliminate cancer. In the group of dr. Joke den Haan we are developing cancer vaccines that are effectively delivered to antigen-presenting cells by the incorporation of single-chain antibodies also known as nanobodies.
In this project, you will produce recombinant protein vaccines that consist of nanobodies and cancer antigens. You will produce nanobodies, purify them using IMAC affinity chromatography and size-exclusion chromatography, test the binding of these vaccines to antigen-presenting cells, and their capacity to activate T cells.
- HLO or MSc degree in molecular biology, biochemistry or pharmaceutics.
- Extensive experience with standard molecular biological and biochemical techniques such as cloning, SDS PAGE, western blot, ELISA, FPLC, HPLC.
- Experience with recombinant protein/nanobody production in E.coli and/or mammalian systems, protein purification by IMAC column affinity chromatography, size-exclusion chromatography, maleimide/NHS-ester conjugations.
- Experience with transfection and transduction of cell lines and primary T cells and analysis by flow cytometry.
- Excellent communication skills in English, both written and verbal.
- Excellent collaboration and organizational skills.
- A flying start to your career in scientific research.
- Plenty of room for your drive to shape tomorrow's healthcare.
- Working on large-scale and in-house research, with motivated colleagues from all over the world.
- You will be employed by Amsterdam UMC Research BV.
- A contract for 12 months, with the intention to extend.
- Salary scale 7M: € 3.064 to € 4.340 gross based on full-time employment (depending on education and experience) and a year-end bonus of 8.3%. Calculate your net salary here.
- Holiday hours: 190.4 per year for fulltime and a possibility to save additional hours.
- Pension accrual with BeFrank, a modern, comprehensible and fairly priced pension.
- For >7 km each way, 100% reimbursement for public transport travel costs and, for private transport, €0.18 per km up to a maximum of 40 km each way.
- Do you prefer walking or cycling? Take advantage of our good bike scheme. Moreover, you will receive a reimbursement of €0.18 per km.
Watch this video with more information about joining Amsterdam UMC Research BV.
The department of Molecular Cell Biology and Immunology (MCBI) at Amsterdam UMC, location VUmc, at the southwest edge of the city carries out research at an internationally competitive level and offers a stimulating and collegial work environment. The research of the department concentrates on immunological questions in the fields of cancer, neuroimmunology, skin immunology, and inflammation. The research focuses on both, fundamental questions aimed at unraveling molecular and cellular mechanisms and translational research focusing on the application of fundamental knowledge to medical questions.
Amsterdam UMC Research BV supports non-profit scientific research. In doing so, we provide researchers with everything they need to excel. Our principal investigators (PIs) and project leaders offer support in the field of project management, finance and human resources. In medical scientific research projects, legal support is also provided.
Watch the video to find out more.
During the publication period, applications will be handled continuously. If the vacancy is filled, it will be closed prematurely.
If you have any questions about this position, please feel free to contact Dr. Joke den Haan or Dr. Hendrik Brink via (020) 444 8080, or via j.denhaan@amsterdamumc.nl or h.j.brink@amsterdamumc.nl.
For more information about the application procedure, please contact Rhiannon Sandfort, recruitment advisor, at r.e.sandfort@amsterdamumc.nl.
A reference check, screening and hiring test may be part of the procedure. Read here whether that applies to you. If you join us, we ask you for a VOG (Certificate of Good Conduct).
Internal candidates will be given priority over external candidates in case of equal suitability.
Acquisition in response to this vacancy will not be considered.