Research Technician – Cell & Molecular Biology with a focus on iPSC technologies

Dr. Harsha Devalla’s group at the Amsterdam UMC is hiring a research technician.

Our lab has established robust approaches for differentiating iPSCs into various cardiac cell types and has spearheaded the development of integrated tissue systems.

Our projects are geared at understanding the molecular basis of heart rhythm disorders, with the ultimate goal of developing personalized therapeutic approaches. By using patient-specific iPSCs, we can recapitulate disease phenotypes in the dish, enabling us to uncover novel mechanisms underlying cardiac arrhythmias and identify potential therapeutic targets.

Your role will include:

• Maintaining iPSC lines and differentiating them into relevant cardiac and neuronal cell types, including cardiomyocytes, and autonomic neurons.
• Carrying out gene editing of iPSCs using CRISPR/Cas9 techniques
• Performing downstream experiments using molecular biology techniques such as DNA/RNA isolation, PCR, qPCR, immunocytochemistry, and viral transduction
• Supporting our laboratory manager with upkeeping of the lab and efficient management of resources such as overseeing inventories, ordering materials and reagents, stockkeeping, and maintaining up-to-date laboratory databases
• Collaborating closely with PhD students and other team members to support their research activities
• Mentoring Bachelor's and Master's students during their internships, guiding them in laboratory techniques and best practices

This position is ideal for someone who enjoys a combination of hands-on research and laboratory management support, with an interest in taking on responsibilities that contribute to the group's overall success.

Essential Educational background and technical skills

• A degree in Life Sciences, Biology, or Medical Laboratory Research (HBO preferred)
• Hands-on experience with cell culture and diverse molecular biology techniques
• Ability to execute experiments with precision, analyze data, and maintain accurate records
• Proficiency in spoken and written English

Desired Technical Experience

• Experience in human iPSC maintenance and differentiation
• Experience with CRISPR-based gene editing techniques

Personal Qualities

• Motivation to contribute to academic research and scientific discovery
• Strong communication skills, with proven ability for teamwork and collaboration
• Excellent problem-solving abilities and adaptability to new techniques and protocols
• Meticulous attention to detail and commitment to maintaining high-quality standards
• Ability to work independently while also functioning effectively as part of a multidisciplinary team