Research technician iPSC models for vascular ocular diseases

As a research technician, you will support the researchers of the Ocular Angiogenesis Group of the Department of Ophthalmology. We focus on vascular diseases of the retina, such as diabetic retinopathy and macular degeneration.

In our research, you will work with vascular organoids derived from patient-specific stem cells (iPSCs). These organoids spontaneously form a capillary network that closely resembles the blood vessels of the eye, offering a more accurate model than previous cell-based systems. To validate the model, we need to confirm that the cells resemble retinal capillaries both structurally and functionally. This involves using advanced microscopy techniques (such as electron microscopy and confocal imaging), flow cytometry, and various functional assays. Next, we will compare iPSC lines from eye patients with control lines or introduce specific gene knockouts.

Our team is part of the national LiveLong Vision consortium, a collaborative initiative of ophthalmologic researchers aimed at combating blindness. Within this consortium, we collaborate with other Dutch universities to develop more advanced models of retinal diseases. If successful in the first 5 years, the program will be extended for up to 10 years.

• You will support us as a research analyst in cell and organoid culture. This involves isolating, characterizing, and maintaining endothelial cells, as well as caring for the organoids. After isolating the endothelial cells, their purity will be checked using flow cytometry. For the organoids, iPS cells will be initiated and differentiated, and the culture will be maintained several times a week.

• You will also be responsible for keeping track of supplies in the culture lab and the storage of our samples in our -80°C freezers and nitrogen tanks.

• Additionally, you may assist with experiments, immunohistochemistry, RNA isolation, qPCR, and western blotting.

• You are an enthusiastic and detail-oriented technician who thrives in a translational research environment, contributing to a young and dynamic team.
• You have experience in cell culture, with a particular focus on human iPSC culturing, differentiation protocols, and assays. Additionally, you are familiar with molecular biology techniques such as fluorescence microscopy, flow cytometry, and western blotting.
• You possess an analytical, solution-oriented mindset, are able to follow protocols accurately, troubleshoot effectively, and find efficient solutions. You can also manage multiple tasks and priorities with ease.
• You have strong communication skills, are service-oriented, and flexible, with the ability to work independently and navigate changing situations.
• You are based in or near Amsterdam, or are willing to relocate to the area.