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Neutrophils meet auto-antibodies: a joint PhD challenge
Do you want to be part of a joint PhD challenge using state-of-the-art cellular and molecular techniques in a translational research project?
- Hours: 32 • 36 uur per week
- Location: Beide locaties Amsterdam UMC
- Contract type: fixed-term
- Salary: € 3.017 - € 3.824
- React until: 2 May 2024
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The project
Rheumatoid arthritis (RA) is a chronic disabling autoimmune disease with an unknown aetiology. The van Egmond and van Baarsen labs both work on unique research angles to better understand the pathophysiology of RA, which now come together in this project.
The van Baarsen lab is the only group in the world where lymph node (LN) biopsies from healthy individuals and patients with or at risk of inflammatory arthritis (IA) are collected and studied. They observed a high number of infiltrating neutrophils in lymph nodes of patients diagnosed with RA.
The van Egmond lab investigates the activation of neutrophils by (auto-)antibodies, and demonstrated that particularly antibodies of the IgA isotype potently activate neutrophils, and induce neutrophil migration. The finding that the presence of IgA autoantibodies predates clinical onset of disease has sparked interest for IgA in the rheumatology research field. Van Baarsen and van Egmond now join forces to investigate the hypothesis that IgA autoantibodies induce the recruitment of neutrophils into the lymph nodes of RA patients, which subsequently steers adaptive immune responses.
Both labs have complementarity tools to tackle this novel hypothesis. The van Baarsen lab has access to unique human samples, and the van Egmond lab recently created novel mouse models. By studying tissue samples from both early stage RA patients as well as (IgA)- autoantibody positive individuals at risk of disease, and through the use of novel and unique technologies, including spatial transcriptomics, this project aims to obtain fundamental new insights in the function of LN neutrophils and their role in RA. Ultimately, gained knowledge may lead to novel therapeutic targets to redirect skewed autoimmune responses in RA and possibly other autoimmune diseases.
The title of the project is "Lymph node neutrophils in inflammatory arthritis: the missing link between innate and adaptive autoimmunity?"
Question about this vacancy?
About your role
As a PhD student on this project you will:
- Establish detailed ex vivo spatial phenotyping of neutrophils in tissue samples (lymph node and synovium) from patients with RA and investigate their relation with clinical parameters including IgA and IgG autoantibody levels;
- Determine neutrophil function and activation by patient autoantibodies;
- Determine the in vivo role of neutrophils and IgA in inflammatory arthritis;
- Work closely with our team and collaborators on this project;
- Author manuscripts submitted to high quality peer-reviewed journals resulting in a PhD thesis;
- Present your work at conferences;
- Be involved in supervising students.
About you
We are looking for a highly motivated and enthusiastic researcher with:
- A master degree Biomedical Sciences, double master Medicine and Biomedical Sciences (SUMMA, DuMa) or equivalent, with affinity for Immunology;
- Experience with cellular responses, molecular biology, and experience with animals (article 9) or patients (MD) is preferred;
- You have a flexible, proactive team spirit but you can also work independently;
- You have excellent communications skills in English, both written and verbal.
Our offer
- A flying start to your career in scientific research.
- Plenty of room for personal development with a wide range of training & development of academic skills
- Working on large-scale and own research, with motivated colleagues
- A contract for 6 months with the prospect of extension for the duration.
- Salary scale OIO: between € 3.017 and € 3.824 gross per month based on a fulltime workweek, depending on qualifications and experience.
- Additional benefits including an 8.3% end-of-year bonus and 8% holiday pay. Calculate your net salary here.
- Your pension is accrued at BeFrank. A modern, understandeble en fair pension
- Excellent accessibility with public transportation and reimbursement of a large portion of your travel expenses. Additionally, we have plenty of parking spaces at the AMC location and a good bicycle scheme.
About your workplace
AMC Medical Research (AMR) is located at location AMC. AMR supports scientific research in non-profit settings. AMR provides researchers with everything they need to excel. For example, AMR offers principal investigators (PIs) and project leaders guidance in the areas of project management, finance and human resources. Legal support is also provided for medical-scientific research projects. Watch the video below for more information.
For this position, we are hiring two PhD students who will elucidate the role of neutrophils in the pathogenesis of rheumatoid arthritis. One of them will work on location VUmc and the other will work on location AMC. Both candidates will frequently interact and collaborate in order to make the project fully translational from bench to bedside.
The two candidates will be working in an international, multidisciplinary team with young and enthusiastic researchers. The project is a joint research program between the group of Dr. Lisa van Baarsen (Amsterdam Rheumatology and immunology Centre (ARC), within the Lab of Experimental Immunology, AMC) and the group of Prof. Marjolein van Egmond (department of Molecular Cell Biology and Immunology, VUmc). The van Egmond lab has ample experience working with human neutrophils, and has established multiple functional assays, and relevant mouse models. The van Baarsen lab has significant experience in collecting and analysing patient samples. Through the synergistic collaboration of the van Egmond and van Baarsen groups, a quick translation of pioneering scientific evidence into a clinical setting can be made.
Amsterdam UMC has an open culture. This means that we hope that everyone feels welcome in our organization and that we strive to offer equal opportunities to everyone. We therefore cordially invite all interested parties to respond to this vacancy. Check out our approach to diversity and inclusion.
What to expect
A job in our beautiful capital city
Working for the favorite employer in healthcare (intermediary 2021)
All colleagues have the same goal: contributing to tomorrow’s care
There is room for development, deepening and broadening of your knowledge
An active staff association that organizes fun activities and arranges discounts on products and services for you
Amsterdam UMC innovates continuously, which requires a flexible attitude from everyone
Two large locations, good for your pedometer!
You have more than 19.000 colleagues, not all of whom you will get to know personally
Meet your colleagues Watch video’s of your future colleagues
Tullip
Amsterdam UMC
Let’s meet!
During the publication period, applications will be handled continuously. If the vacancy is filled, it will be closed prematurely.
If you have any questions about this position, please feel free to contact Lisa van Baarsen, via e.g.vanbaarsen@amsterdamumc.nl.
For more information about the application procedure, please contact Rhiannon Sandfort, Recruitment Advisor, via r.e.sandfort@amsterdamumc.nl.
A reference check, screening and hiring test may be part of the procedure. Read here whether that applies to you. If you join us, we ask you for a VOG (Certificate of Good Conduct).
Internal candidates will be given priority over external candidates in case of equal suitability.
Acquisition in response to this vacancy is not appreciated.