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PhD student Transcytosis in Barrier Endothelium

Unravel molecular mechanisms involved in endothelial barrier breakdown
PhD student Transcytosis in Barrier Endothelium
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  • Aantal uur: 36 uur per week
  • Werklocatie: Locatie AMC
  • Contractvorm: bepaalde tijd
  • Salaris: € 2.570 - € 3.271
  • Reageer tot en met: 24 mei 2022
Solliciteer direct Reageer binnen 6 dag(en)
Wat ga je onderzoeken?

To maintain a proper function of the central nervous system (CNS), the blood-brain barrier (BBB) and the blood-retinal barrier (BRB) are required to be intact to prevent disturbances of the carefully composed environment for these sensitive organs. These physiologically analogous barriers are formed by a continuous, non-fenestrated endothelial monolayer with a highly restrictive excretion of molecules.

Most attention has been paid to the importance of tight junctions, but recent developments have highlighted the regulation of transcytosis as a fundamental factor in barrier integrity. Endothelial transcytosis is usually a caveolae-dependent process that combines receptor-mediated endocytosis, vesicle trafficking via actin cytoskeletal remodeling, vesicle fusion and exocytosis.

Vragen over deze functie?
Tanja Hart Tanja Hart +31621603178 Recruitment adviseur
Wat ga je doen?

In the present project, you will investigate the proteins involved in transcytosis in more detail, using specific pathway inhibitors, mass spectrometry and other molecular techniques. To more specifically track the endocytosis and exocytosis of tracers, you will use confocal imaging and total internal reflection fluorescence (TIRF) microscopy in real-time in living cells. Barrier integrity will be monitored with a CellZscope system.

    Wat neem je mee?

    We are looking for an enthusiastic PhD student with the following characteristics:

    • Master's degree in Biomedical Sciences, Life Science Technology, Bio-Pharmaceutical Sciences or other closely related discipline.
    • Demonstrated experience in cell and molecular biology, cell signaling and confocal microscopy.
    • Affinity with the subject of study.
    • Fluent in English (written and verbal).
    • Good writing skills.
    • Excellent communication and collaboration skills.
    Wat bieden we jou?
    • A jump start to your career in research work.
    • All te space for you to contribute shaping the care of tomorrow.
    • The position is for 4 years. We first offer a fixed term contract for 1 year, which under normal circumstances will be prolonged by 3 years (after evaluation). 
    • Besides a good basic salary, you will also receive an 8.3% year-end bonus and 8% holiday allowance. 
    • In addition to excellent accessibility by public transport, AMC also has a sufficient number of parking spaces for employees.
    • Pension is accrued at Be Frank. 
    • An active personnel association and the Young Amsterdam UMC association, both of which organise fun (sports) activities and events.

    For more information about our terms of employment, please visit our website.

    Waar ga je werken?

    The Ocular Angiogenesis group, headed by Prof. Reinier Schlingemann and Dr. Ingeborg Klaassen, is part of the Department of Ophthalmology and housed in the Department of Medical Biology. Within our team you work closely together with other PhD students from our own group and collaborative groups. Within the Amsterdam UMC, we have several collaborations with other researches within the field of vascular research, cancer research and neurosciences. Furthermore, we collaborate with numerous national and international groups.

    In our department the latest technical advances in imaging, such as high-resolution confocal imaging, correlative electron microscopy and live-cell imaging are available.

    More information can be found on our website: ocular-angiogenesis.nl

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    Applications will be considered continuously during the publication period. If the vacancy is filled, it will be closed prematurely. 

    For more information on the project, contact Dr. Ingeborg Klaassen via i.klaassen@amsterdamumc.nl 

    For more information about the application process please contact Tanja Hart, Recruitment adviser, via t.hart@amsterdamumc.nl or via 06-21603178.

    A reference check, screening and hiring test may be part of the procedure. Read here whether this also applies to you.

    If you join us, we ask for a VOG (Certificate of Good Conduct)