We are looking for a motivated PhD student who has been trained in cell culture and molecular techniques (preferred background in (molecular) biology, biochemistry or cell biology), who is willing to join our research team to work on this project. You will use primary human endothelial cells in combination with biochemical and cell biological techniques, including (super resolution) live-cell confocal microscopy.
What you are going to do
Within the department of Physiology at Amsterdam UMC, location VUmc, we focus on basic and translational research in the context of cardiovascular disease. In our group, we are particularly interested in the molecular mechanisms that control cell-cell contact between human endothelial cells. These cells line all our blood vessels and are criticial ‘gatekeepers’ that control coagulation and the transport of fluids and immune cells from the circulation to the tissues and vice versa. This is important during inflammation, for example, and since inflammation is at the basis of cardiovascular disease, understanding its underlying mechanisms is key to the future development of effective therapies.
The regulation of cell-cell contact in endothelium is a major research topic in the group. Since this cell-cell contact requires the cytoskeleton, we focus on a group of proteins, the Rho-like small GTPases, that are central regulators of the actin cytoskeleton. Recently, we identified GTPases that are positive or negative modulators of endothelial cell-cell contact. We also studied the regulation of key Rho GTPases by post-translational modification, in particular ubiquitination.
In this project, we aim to study Rho GTPase regulation and modification 2D and 3D models for the microcirculation. We will use human primary-, or iPSC-derived endothelial cells in the absence or presence of flow and in resting or activated (i.e inflammation) conditions. Gain and loss of function studies as well as manipulation of signaling pathways in combination with live cell confocal imaging will be used to generate new fundamental insights on the control of endothelial integrity.
What we expect from you
Prerequisites are as follows:
We offer you ample opportunity for development, deepening and broadening, additional training and a place to grow! Working at AUMC means working in an inspiring and professional environment where development is encouraged in every respect.
Where you are going to work
You will work in a team of PhD students and postdocs that all study various aspects of endothelial and vascular biology, such as metabolic disorders, obesity-related vascular dysfunction and aging. In addition, the department has strong research lines in the fields of cardiomyocyte contractility and studies the crosstalk between heart muscle cells and the endothelium. The dept of Physiology is part of the Amsterdam UMC Research Center ‘Amsterdam Cardiovascular Sciences and of the AUMC ‘hartcentrum’. This means that the project is well embedded in department as well as in the larger AUMC research institute.
For more information about this position, you can contact Peter Hordijk, email@example.com
For more information about the application procedure please contact Tanja Hart, Recruitment adviser, via firstname.lastname@example.org or via 06-21603178.
A reference check, screening and hiring test may be part of the procedure. Read hier whether this also applies to you.
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